The Bombay Technologist

Polymeric Delivery V^hide for Gene Therapy

Kaushik Mishra

Keywords: Polyplex, Drue, Delivery System, Bio-Medical Applications, Polymer, Pharmacology.

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Abstract

Synthetic gene therapy vectors must be designed to safely and efficiently escort DNA from outside the cell to the nucleus and to overcome several barriers that are obstacles to internalization, escape from endocytic vesicles, movement through the cytoplasm and transport into the nucleus. Non-viral transaction systems based on the complexes of DNA and polycations Cpolyplexes') are evaluated with respect to their effectiveness, toxicity and cell type dependence in a variety of models. Overall, the article demonstrates good potential of structurally diverse polyplex systems as transaction reagents with relatively low cytotoxicity.